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The ancient herbal books, and literatures were reviewed, and the textual research and origin of Caryophylli flos were explored from the differences between Caryophylli flos and Caryophylli fructus, the alias of Caryophylli flos and Caryophylli fructus, the origin and introduction of Caryophylli flos, and its efficacy and indications. The results indicated that Caryophylli flos and Caryophylli fructus were different from each other in terms of synonyms, chemical components and pharmacological effects. Caryophylli flos was brought to China as a spice in the Wei, Jin, Southern and Northern Dynasties, and the efficacy of Caryophylli flos was almost same in ancient and modern time. Caryophylli flos is widely used in digestive and dental diseases, and Caryophylli flos is also one of the seven natural spices, which can be used for food preservation.
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The present study aimed to regulate the market circulation of Caryophylli Flos and formulate standards for commodity specifications and grades of Caryophylli Flos. Market survey was carried out in four major medicinal material markets with 48 samples of Caryophylli Flos collected. The property, 100-seed weight, impurity percentage, moisture, and eugenol content in Caryophylli Flos of different specifications from different producing areas were determined and analyzed. The results showed that 27.1% of the samples surveyed on the markets did not meet the requirements of Chinese Pharmacopoeia(2020 edition). The 100-seed weight and the property are important factors for the classification of Caryophylli Flos specifications. There were significant differences in the property, 100-seed weight, impurity percentage, and eugenol content in Caryophylli Flos samples of different specifications from different producing areas, and also differences in the proportions of different specifications in Caryophylli Flos samples from different producing areas. The African-originated Xiaohong(medium grade) and Guangxi-originated Xiaohong(medium grade) accounted for 70% and 66.7% respectively, the Indonesian-originated Dahong(top grade) for 56.2%. In conclusion, there are many problems in the circulation of Caryophylli Flos at present, mainly including the loss of origin information, no standards for specifications, non-implementation of grade standards, excessive impurities, and no evidence for authenticity identification. According to the classification of Caryophylli Flos specifications in this study, the average eugenol content of Xiaohong is significantly higher than the Dahong by 4.74%.
Subject(s)
China , Drugs, Chinese Herbal/analysis , IndonesiaABSTRACT
Objective: In this study, network pharmacology techniques and methods were used to screen the material basis, targets and pathways of the interaction between the pungent-aromatic herbs (Citri Reticulatae Pericarpium, Caryophylli Flos, and Foeniculi Fructus) and olfactory receptor. To explain the molecular mechanism of the expression of pungent properties of the pungent-aromatic herb. Methods: TCMSP database was used to collect the chemical components of Citri Reticulatae Pericarpium, Caryophylli Flos, and Foeniculi Fructus, in order to screen out the effective components and predict the related targets. All the proteins related to olfactory receptors were collected from UniProt database. The selected targets and ORs proteins were uploaded to the online STRING database for protein interaction analysis. The interaction network was drawn by using the software of Cytoscape software, and the key targets was further analyzed by GO functional enrichment analysis and KEGG pathway enrichment analysis. Results: Five active components of Citri Reticulatae Pericarpium, five of Caryophylli Flos and two of Foeniculi Fructus, 224 targets and 515 olfactory receptors were obtained. Protein interaction network showed 25 targets (such as GNB2, CHRM2, CHRM3, GNGT2, ADRA2A) were the key targets for ORs of three pungent-aromatic herbs. The enrichment analysis of GO and KEGG identified 31 biological processes and 78 signaling pathways. Conclusion: Olfactory receptors are mainly activated by volatile oil components of the pungent-aromatic herb. And the classical cAMP signaling pathway may not be the only one after the olfactory receptor activated, which may be involved in the expression of pungent properties of the pungent-aromatic herb together with several other signaling pathways, so as to produce "pungent flavor".
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Eugenia caryophyllata,a plant of Myrtle family,has a long history of cultivation and application in China.It is a medicinal and edible homologous plant with rich pharmacological activities.At present,there have been reports on the pharmacological effects and chemical components of Caryophylli Flos,but the clinical research progress was less.Therefore,this paper was to summarize the studies on the chemical composition and pharmacological action of Caryophylli Flos,through literature review,this paper introduced the record of Caryophylli Flos in traditional Chinese medicine(TCM) and Mongolian medicine,the application frequency of Caryophylli Flos in traditional Mongolian medicine and its compatibility of drug-pair were analyzed with computer software.In addition,the use of Caryophylli Flos in Mongolian medicine was discussed from the aspects of their medicinal property,functional treatment and clinical application,so as to provide reference for the subsequent studies on Caryophylli Flos.
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Objective: To establish an HPLC fingerprint of Caryophylli flos. to provide reference for the overall quality evaluation of the herb. Methods: The fingerprint of Caryophylli flos. was established by HPLC, and eugenol was used as the reference peak for the exploration of HPLC conditions. The fingerprint of Caryophylli flos. was established with acetonitrile-0. 2% phosphoric acid as the mobile phase with gradient elution. Results: A total of 17 characteristic common peaks were obtained by HPLC analysis, and among them, five common constituents including eugenol, ferulic acid, quercetin, kaempferol and isorhamnetin were calibrated. The similari-ties of chromatographic fingerprint of Caryophylli flos. from 10 different origins were above 0. 900, which was in line with the basic re-quirement of fingerprint similarity evaluation. Conclusion: The study provides experimental basis for improving the quality evaluation system of Caryophylli flos. , and provides reference for a comprehensive quality assessment of Caryophylli flos. .
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Objective: Using the improved volatile oil extractor to extract the essential oil of Caryophylli Flosand emulsified fragrance water. To research the correlations of the emulsified fragrance water from different condensing temperature and different temperature with the physicochemical parameters under the different temperature of essential oilin Caryophylli Flos;The results help a lot for the optimization of extraction process parameters of essential oil in Caryophylli Flos. Methods: Using surface tension tester, determination of viscometer to measure viscosity, surface tension, interfacial tension under different temperature of essential oil density in Caryophylli Flos, using stability analyzer to determine the emulsified fragrance water stability within 1 h and particle sizeunder different extraction temperature and different collecting temperature. Using multivariate data analysis to research the relationship between the essential physical and chemical parameters with emulsified fragrance water stability.Results: Relativedensity, viscosity, and surface tension reduced as the temperature of essential oil in Caryophylli Flos increased gradually, interfacial tension with increased first and then decreased as temperature increased. Turbiscan Stability Index(TSI) of the volatile oil emulsified fragrance water was negative correlation with the condensation temperature and interfacial tension, and was positive correlation with collecting temperature, density, viscosity, and surface tension. Conclusion: During the extraction process, temperature, interfacial tension, and condensation temperature of volatile oil in Caryophylli Floswere collected with aromatic water, and TSI hascertain relevance. Through controlling the collected temperature, condensation temperature, interfacial tension, and oil/water separation speed of volatile oil emulsified fragrance water have been changed and the guidance to the extraction process has been provided.
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Objective To establish a quality standard for Aiweixin oral liquid. Methods TLC was applied to the identification of Caryophylli Flos and Crocin in Aiweixin oral liquid. Eugenol was analyzed by GC. Results TLC of Caryophylli Flos and Crocin had distinct separation of characteristic spots and there was no interference in negative comparison. The linear response ranges of Eugenol were between 0.052 17-2.086 8μg (r=0.999 9). The samples were steady within 24 h. The average recovery of eugenol was 95.7%, RSD=1.4% (n=6). Conclusion The method is simple, accurate and reliable, and can be used for the quality control of Aiweixin oral liquid.